CCT Mrna and CREB phosphorylation upregulated by xxx in rat hippocampus and XXXX.

Yu-cang Du, Kan-yan Xu, Ying Xiong,Ming Dong, Xiu-Fang CHEN, Shanghai Institute of Biochemistry, Shanghai Institute of Physiology, Chinese Academy of Sciences,Shanghai 200031,China

CTP: phosphocholine cytdylyltransferase(CCT) is a key enzyme in vivo phospholipide synthesis.So it might play an important role in the pathogensis of neurodegenerative disease lide AD. XXXXXXXXXXX long-term memory depended on cyclic AMP-response element binding protein, CREB. CREB phosphorylation is essential for it’s transcriptional activity. XXXpeptide ZNCPR and it’s analogs were indicated to have prtent memory-enhacing activity, facilitate neurite enongation and prolongate cell ageing, the peptide-up regulation of CCT and CREB mRNA and was studied in this paper. ①xxx xxxxxxxxxxxxxxxx from Western blot with specific anti-phosphorylated CREB antibody indicated.xxxx.②By using DD-PCR and 5’ rapid amplification of cDNA ends approaches, xxxxx xxxx cDNA xxxxx rats CCT-βwas obtained. ③The study xx xxxx xxxxxx with reverse transcriptin PCR showed that the gene was abundant in rat brain. Based on xxx, we now know that xxx xxxx xxxxx xxxxx xxxxxxxx